Description
Triglyceride Assay Kit (Colorimetric) (BA0152) (BA0152)
The Triglyceride Assay Kit (SKU: BA0152) provides a simple, direct and automation-ready procedure for measuring triglyceride concentration. Triglyceride is the main constituent of vegetable oil and animal fat and plays an important role as an energy source and transporter of dietary fat; high blood levels have been linked to atherosclerosis, heart disease and pancreatitis. This assay uses a single working reagent that combines triglyceride hydrolysis and glycerol determination in one step, in which a dye reagent is oxidised to form a coloured product. The colour intensity at 570 nm is directly proportional to the triglyceride concentration in the sample. The optimised formulation enhances reagent and signal stability and the assay uses as little as 10 uL of sample.
| Product Name: | Triglyceride Assay Kit (Colorimetric) (BA0152) |
| SKU: | BA0152 |
| Detection Method: | Colorimetric |
| Detection Range: | 0.01 - 1.0 mmol/L (0.88 - 88.5 mg/dL) triglyceride |
| Sample Type: | Serum, plasma and other biological samples |
| Species Reactivity: | All |
| Assay Time: | 30 minutes |
| Kit Size: | 200 Assays |
| Equipment Required: | Microplate reader |
| Storage: | Store all components at -20 C. |
| Shelf Life: | 12 months after receipt |
| Shipping: | Gel Pack |
A single-reagent enzymatic assay for the quantitative colorimetric determination of triglyceride at 570 nm. Triglyceride is hydrolysed and the released glycerol is determined through a coupled reaction that oxidises a dye reagent to a coloured product. The procedure adds a single working reagent and reads after a 30 min room-temperature incubation.
- Sensitive and accurate, using as little as 10 uL of sample
- Linear detection range 0.01 - 1.0 mmol/L (0.88 - 88.5 mg/dL) triglyceride
- Simple single-working-reagent format with a 30 min incubation
- Improved reagent and signal stability
- Direct measurement of triglyceride in biological samples such as serum and plasma
- Studies of the effects of drugs on triglyceride metabolism
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Equilibrate all components to room temperature, keeping thawed Lipase and Enzyme Mix refrigerated or on ice. |
| 2 | Prepare standards by diluting the Standard to 1.0, 0.6, 0.3 and 0 mmol/L. Transfer 10 uL of each into wells of a clear 96-well plate. |
| 3 | Prepare samples: dilute serum and plasma 5-fold in water; solubilise cells and solid samples in 5% Triton X-100. Transfer 10 uL of each sample into separate wells. |
| 4 | Prepare Working Reagent per well by mixing 100 uL Assay Buffer, 2 uL Enzyme Mix, 5 uL Lipase, 1 uL ATP and 1 uL Dye Reagent. Transfer 100 uL to each well and tap to mix. |
| 5 | Incubate for 30 min at room temperature and read optical density at 570 nm (550-585 nm). |
Subtract the water blank from the standard values and plot against triglyceride concentration to determine the slope by linear regression. Calculate [Triglyceride] = ((ODSAMPLE - ODWATER) / Slope) x n (mmol/L), where n is the dilution factor (n = 5 for serum or plasma). One mmol/L triglyceride equals 88.5 mg/dL.
| Component | Quantity | Storage |
| Assay Buffer | 24 mL | -20 C |
| ATP | 250 uL | -20 C |
| Dye Reagent | 220 uL | -20 C |
| Enzyme Mix | 500 uL | -20 C |
| Lipase | 1000 uL | -20 C |
| Standard (100 mmol/L Triglyceride) | 100 uL | -20 C |
