Description
Copper Assay Kit (Colorimetric) (BA0035) (BA0035)
The Copper Assay Kit (Colorimetric) (SKU: BA0035) is designed to measure copper with no or minimal sample treatment. Copper is an essential trace element whose enzymes play important roles in iron and catecholamine metabolism, free-radical scavenging and the synthesis of haemoglobin, elastin and collagen. Low copper levels are associated with anaemia, hypotonia and bone changes, while copper levels are a key diagnostic indicator of Wilson's disease and microcytic hypochromic anaemia. This kit uses a chromogen that forms a coloured complex specifically with copper ions; the intensity measured at 359 nm is directly proportional to the copper concentration. The optimised formulation substantially reduces interference from substances in raw samples. The simple procedure is readily automated for high-throughput work and can be run in 96-well plate or cuvette format. Note that metal chelators such as EDTA interfere with the assay.
| Product Name: | Copper Assay Kit (Colorimetric) (BA0035) |
| SKU: | BA0035 |
| Detection Method: | Colorimetric; chromogen complex with copper ions measured at 359 nm |
| Detection Range: | 7 µg/dL (1.0 µM) to 300 µg/dL (47 µM) copper |
| Sample Type: | Biological, environmental, food and beverage samples |
| Species Reactivity: | All |
| Assay Time: | 5 min incubation |
| Kit Size: | 250 Assays |
| Equipment Required: | Microplate reader |
| Storage: | Store all reagents at 4°C. |
| Shelf Life: | 6 months after receipt. |
| Shipping: | Room Temperature |
A colorimetric assay for the quantitative determination of copper with minimal sample treatment. A chromogen forms a coloured complex specifically with copper ions; the intensity measured at 359 nm is directly proportional to the copper concentration. Linear detection range is 7 µg/dL (1.0 µM) to 300 µg/dL (47 µM).
- Sensitive and accurate: linear detection range 7 to 300 µg/dL copper.
- Simple and high-throughput: readily automated in 96-well plates for thousands of samples per day.
- Improved reagent stability and versatility: enhanced reagent and signal stability; cuvette or 96-well plate assay.
- Copper in biological, environmental, food and beverage samples.
- Drug discovery and pharmacology: effects of drugs on copper metabolism.
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Standards: transfer 100 µL dH2O into a tube labelled Blank and, in another tube labelled Standard, mix 20 µL 1.5 mg/dL Standard with 80 µL dH2O (final 300 µg/dL Cu2+). |
| 2 | Samples: transfer 100 µL of each sample into separate tubes. Add 35 µL Reagent A (trichloroacetic acid) to each and vortex; for protein-containing samples, centrifuge 2 min at 14,000 rpm and use the clear supernatant. Transfer 100 µL Blank, Standard and Sample into separate wells of a clear flat-bottom 96-well plate. |
| 3 | Prepare Working Reagent per well by mixing 5 µL Reagent B and 150 µL Reagent C. Transfer 150 µL Working Reagent to each well and tap to mix thoroughly. |
| 4 | Incubate 5 min at room temperature and read the optical density at 356-362 nm (peak absorbance at 359 nm). |
| 5 | Cuvette format: transfer 400 µL Standards and Samples into cuvettes, add 600 µL Working Reagent, mix, incubate 5 min and read at 356-362 nm. |
[Copper] = [(ODsample - ODblank) / (ODstandard - ODblank)] x 300 (µg/dL). Conversions: 100 µg/dL Cu equals 15.5 µM, 0.0001% or 1 ppm.
| Component | Quantity | Storage |
| Reagent A | 10 mL | 4°C |
| Reagent B | 1.5 mL | 4°C |
| Reagent C | 40 mL | 4°C |
| Copper Standard (1.5 mg/dL Cu2+) | 1 mL | 4°C |
