The SERPING1 Antibody (CAB1717) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By binding specifically to the Serping1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in immunology and inflammatory disorders.Serping1, also known as C1 inhibitor, is a key player in controlling the complement system, which is essential for immune response regulation and defense against pathogens.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SERPING1 Antibody
SKU:
CAB1717
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C1IN, C1NH, HAE1, HAE2, C1INH, SERPING1
Positive Sample:
Human plasma
Cellular Localization:
Secreted.
Calculated MW:
55kDa
Observed MW:
100kDa
This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its encoded protein, C1 inhibitor, inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. It is synthesized in the liver, and its deficiency is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform.
Purification Method
Affinity purification
Gene ID
710
RRID
AB_2763768
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Human plasma, using SERPING1 Rabbit pAb (CAB1717) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using SERPING1 Rabbit pAb (CAB1717) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
