[KO Validated] Caspase-9 Rabbit Polyclonal Antibody (CAB0281)

(No reviews yet) Write a Review
お見積り依頼
SKU:
CAB0281
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, Caspase-9
Research Use:
Cell Death
€0.00
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

[KO Validated] Caspase-9 Antibody (CAB0281)

The [KO Validated] Caspase-9 Antibody (CAB0281) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced by Assay Genie and raised in rabbits, is highly specific for human caspase-9 and is validated for use in Western blot applications. Caspase-9 is a key player in the intrinsic apoptotic pathway, acting as an initiator caspase that is activated in response to various cellular stress signals. Dysregulation of caspase-9 activity has been implicated in numerous diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] Caspase-9 Antibody
SKU:CAB0281
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:VGAL ESLR GNAD LAYI LSME PCGH CLII NNVN FCRE SGLR TRTG SNID CEKL RRRF SSLH FMVE VKGD LTAK KMVL ALLE LAQQ DHGA LDCC VVVI LSHG CQAS HLQF PGAV YGTD GCPV SVEK IVNI FNGT SCPS LGGK PKLF FIQA CGGE QKDH GFEV ASTS PEDE SPGS NPEP DATP FQEG LRTF DQLD AISS LPTP SDIF VSYS TFPG FVSW RDPK SGSW YVET LDDI FEQW AHSE DLQS LLLR VANA VSVK GIYK QMPG CFNF LRKK LFFK TS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:100 - 1:500
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, Caspase-9
Positive Sample:HepG2, 293T
Cellular Localization:Apoptosome, Cytoplasm, Cytosol, Mitochondrion, Nucleus.
Calculated MW:46kDa
Observed MW:35kDa/37kDa/47kDa

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID842
RRIDAB_2757093
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Caspase-9 Rabbit pAb (CAB0281) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver tissue using Caspase-9 Rabbit pAb (CAB0281) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using Caspase-9 Rabbit pAb (CAB0281) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using Caspase-9 Rabbit pAb (CAB0281) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

View AllClose

Related Products