[KO Validated] CETN2 Rabbit Polyclonal Antibody (CAB5397)

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SKU:
CAB5397
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse, Rat
Synonyms:
CALT, CEN2, N2
Research Use:
Cell Cycle
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Description

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[KO Validated] CETN2 Antibody (CAB5397)

The [KO Validated] CETN2 Antibody (CAB5397) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications.Centrin-2 is a key player in centriole biology, where it plays a crucial role in maintaining centriole structure and function. Dysregulation of centriole duplication has been implicated in various diseases, including cancer and ciliopathies. By using the Centrin-2 Polyclonal Antibody, researchers can detect and analyze Centrin-2 protein expression in different cell types, providing insights into its role in cellular processes and disease mechanisms.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] CETN2 Antibody
SKU:CAB5397
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MASN FKKA NMAS SSQR KRMS PKPE LTEE QKQE IREA FDLF DADG TGTI DVKE LKVA MRAL GFEP KKEE IKKM ISEI DKEG TGKM NFGD FLTV MTQK MSEK DTKE EILK AFKL FDDD ETGK ISFK NLKR VAKE LGEN LTDE ELQE MIDE ADRD GDGE VSEQ EFLR IMKK TSLY
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CALT, CEN2, N2
Positive Sample:HeLa, 293T, Mouse brain, Rat brain
Cellular Localization:Cytoplasm, Nucleus, Centriole, Centrosome, Cytoskeleton, Microtubule Organizing Center.
Calculated MW:20kDa
Observed MW:20kDa

Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome.

Purification MethodAffinity purification
Gene ID1069
RRIDAB_2766206
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] CETN2 Rabbit pAb (CAB5397) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of lysates from wild type (WT) and CETN2 knockout (KO) HeLa cells, using [KO Validated] CETN2 Rabbit pAb (CAB5397) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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