[KO Validated] Caspase-8 Rabbit Monoclonal Antibody (CAB19549)

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SKU:
CAB19549
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
Antibody Type:
Monoclonal Antibody
Synonyms:
CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8
Research Use:
Cell Death
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Description

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[KO Validated] Caspase-8 Monoclonal Antibody (CAB19549)

The [KO Validated] Caspase-8 Monoclonal Antibody (CAB19549) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, validated in knockout (KO) models, is highly specific to caspase 8 and is ideal for use in various experimental techniques such as Western blot analysis. By targeting caspase 8, researchers can gain valuable insights into the mechanisms of cell death and survival, providing a deeper understanding of diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:[KO Validated] Caspase-8 Monoclonal Antibody
SKU:CAB19549
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC0028
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:EELC GVMT ISDS PREQ DSES QTLD KVYQ MKSK PRGY CLII NNHN FAKA REKV PKLH SIRD RNGT HLDA GALT TTFE ELHF EIKP HDDC TVEQ IYEI LKIY Q
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8
Positive Sample:Jurkat, Hep G2, HeLa
Cellular Localization:Cytoplasm, Nucleus .
Calculated MW:55kDa
Observed MW:57kDa

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Purification MethodAffinity purification
Gene ID841
RRIDAB_2862662
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] Caspase-8 Rabbit mAb (CAB19549) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Product ImageWestern blot analysis of lysates from wild type (WT) and caspase-8 knockout (KO) HeLa cells using [KO Validated] Caspase-8 Rabbit mAb (CAB19549) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:5000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 5% nonfat dry milk in TBST. Exposure time: 60s. The data were kindly provided by Dr. Feng Shao, NIBS
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