[KO Validated] Cytokeratin 8 Rabbit Polyclonal Antibody (CAB1024)

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SKU:
CAB1024
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse
Synonyms:
K8, KO, CK8, CK-8, CYK8, K2C8, CARD2, Cytokeratin 8 (KRT8)
Research Use:
Immunology
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Description

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[KO Validated] Cytokeratin 8 Antibody (CAB1024)

The [KO Validated] Cytokeratin 8 Antibody (CAB1024) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically targets KRT8 and has been validated for use in various applications, including Western blot analysis.KRT8 is involved in maintaining the structural integrity of epithelial cells and has been implicated in cell signaling pathways, cell migration, and stress responses.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] Cytokeratin 8 Antibody
SKU:CAB1024
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSIR VTQK SYKV STSG PRAF SSRS YTSG PGSR ISSS SFSR VGSS NFRG GLGG GYGG ASGM GGIT AVTV NQSL LSPL VLEV DPNI QAVR TQEK EQIK TLNN KFAS FIDK VRFL EQQN KMLE TKWS LLQQ QKTA RSNM DNMF ESYI NNLR RQLE TLGQ EKLK LEAE LGNM QGLV EDFK NKYE DEIN KRTE MENE FVLI KKDV DEAY MNKV ELES RLEG LTDE INFL RQLY EEEI RELQ SQIS DTSV VLSM DNSR SLDM DSII AEVK AQYE DIAN RSRA EAES MYQI KYEE LQSL AGKH GDDL RRTK TEIS EMNR NISR LQAE IEGL KGQR ASLE AAIA DAEQ RGEL AIKD ANAK LSEL EAAL QRAK QDMA RQLR EYQE LMNV KLAL DIEI ATYR KLLE GEES RLES GMQN MSIH TKTT SGYA GGLS SAYG GLTS PGLS YSLG SSFG SGAG SSSF SRTS SSRA VVVK KIET RDGK LVSE SSDV LPK
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:2000 - 1:4000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:K8, KO, CK8, CK-8, CYK8, K2C8, CARD2, Cytokeratin 8 (KRT8)
Positive Sample:Mouse large intestine
Cellular Localization:Cytoplasm, Nucleus, Nucleus Matrix, Nucleoplasm.
Calculated MW:54kDa/56kDa
Observed MW:54kDa

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Purification MethodAffinity purification
Gene ID3856
RRIDAB_2757765
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Mouse large intestine, using Cytokeratin 8 (KRT8) Rabbit pAb (CAB1024) at 1:4000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Cytokeratin 8 (KRT8) Rabbit pAb (CAB1024) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of MCF7 cells using Cytokeratin 8 (KRT8) Rabbit pAb (CAB1024) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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