Description
Stable-Lux Luciferase Assay System (ASRV00014)
Stable-Lux Luciferase Assay System is a homogeneous reagent solution designed for ultra-high sensitivity and stable quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells. This system is optimized for batch processing of 96- and 384-well plates, delivering long-lived luminescence when added directly to cultured cells. The homogeneous assay provides a highly stable luminescent response, making it ideal for high-throughput applications. Throughput rates of several thousand samples per hour can be achieved with high reproducibility under standard laboratory conditions.
| Product Name: | Stable-Lux Luciferase Assay System |
| SKU: | ASRV00014 |
| Size: | 10ml, 10×10ml, 100ml |
| Detection Principle: | Luminescence |
| Step | Protocol |
| 1 | Remove the cell culture plate to be tested from the incubator and equilibrate at room temperature for 30 min. |
| 2 | Add equal amount of Stable-Lux Luciferase Detection Reagent which is equal to the volume of the cell culture to be tested. For example, when using a 96-well culture plate, add 100 μl Stable-Lux Luciferase Detection Reagent to 100 μl cell culture to be tested. |
| 3 | Incubate at room temperature for 5 minutes to lyse the cells. |
| 4 | Detect luminescence. |
| Step | Protocol |
| Thawing | Incubate the Stable-Lux Luciferase Assay Buffer at 2 to 8℃ or room temperature to thaw. The product can also be incubated at 22℃ in a water bath but the temperature should not exceed 25℃. |
| Preparation of Stable-Lux Luciferase Detection Reagent | Add the entire bottle of thawed Stable-Lux Luciferase Buffer into the Stable-Lux Luciferase Substrate. Gently invert and mix it well for 3 to 5 times until the substrate is dissolved thoroughly. |
| Equilibrate to room temperature | Equilibrate the Stable-Lux Luciferase Detection Reagent to room temperature before use. |
| Mix | Gently invert 5 times before use, to mix the solution evenly. |
| Product | Code | Contents |
| Stable-Lux Luciferase Assay System (10ml) | ASRV00014-10 | Stable-Lux Luciferase Assay Buffer (10ml) Stable-Lux Luciferase Assay Substrate (lyophilized) 1 Vial |
| Stable-Lux Luciferase Assay System (10x10ml) | ASRV00014-10x10 | Stable-Lux Luciferase Assay Buffer (10x10ml) Stable-Lite Luciferase Assay Substrate (lyophilized) 10 Vials |
| Stable-Lux Luciferase Assay System (100ml) | ASRV00014-100 | Stable-Lux Luciferase Assay Buffer (100ml) Stable-Lite Luciferase Assay Substrate (lyophilized) 1 Vials |
