Description
Bio-Lux Luciferase Assay System (ASRV00013)
The Bio-Lux Luciferase Assay System is a robust, highly sensitive, and homogeneous reagent solution specifically designed for the stable quantitation of firefly (Photinus pyralis) luciferase reporter gene expression in demanding applications like Reporter Bioassays. This system is optimized for batch processing of 96- and 384-well plates, delivering long-lived luminescence when added directly to cultured cells. The Bio-Lux system contains high-purity Luciferin and an optimized reaction reagent formulation. This chemistry results in enhanced stability and greater tolerance to sample components and environmental factors than traditional luciferase assay reagents. The system is supplied as a two-component kit (solution and substrate) that is mixed prior to use.
| Product Name: | Bio-Lux Luciferase Assay System |
| SKU: | ASRV00013 |
| Size: | 10ml / 10×10ml / 100ml |
| Detection Principle: | Luminescence |
| Step | Protocol |
| 1 | Remove the cell culture plate to be tested from the incubator and equilibrate at room temperature for 30 min. |
| 2 | Add equal amount of Bio-Lux Luciferase Detection Reagent which is equal to the volume of the cell culture to be tested. For example, when using a 96-well culture plate, add 100 μl Bio-Lux Luciferase Detection Reagent to 100 μl cell culture to be tested. |
| 3 | Incubate at room temperature for at least 3 minutes to lyse the cells. |
| 4 | Detect luminescence. |
| Step | Protocol |
| Thawing | Incubate the Bio-Lux Luciferase Assay Buffer at 2 to 8℃ or room temperature to thaw. The product can also be incubated at 22℃ in a water bath but the temperature should not exceed 25℃. |
| Preparation of Bio-Lux Luciferase Detection Reagent | Add the entire bottle of thawed Bio-Lux Luciferase Buffer into the Bio-Lux Luciferase Substrate. Gently invert and mix it well for 3 to 5 times until the substrate is dissolved thoroughly. |
| Equilibrate to room temperature | Equilibrate the Bio-Lux Luciferase Detection Reagent to room temperature before use. |
| Mix | Gently invert 5 times before use, to mix the solution evenly. |
| Product | Code | Contents |
| Bio-Lux Luciferase Assay System (10ml) | ASRV00013-10 | Bio-Lux Luciferase Assay Buffer (10ml) Bio-Lux Luciferase Assay Substrate (lyophilized) 1 Vial |
| Bio-Lux Luciferase Assay System (10x10ml) | ASRV00013-10x10 | Bio-Lux Luciferase Assay Buffer (10x10ml) Stable-Lite Luciferase Assay Substrate (lyophilized) 10 Vials |
| Bio-Lux Luciferase Assay System (100ml) | ASRV00013-100 | Bio-Lux Luciferase Assay Buffer (100ml) Stable-Lite Luciferase Assay Substrate (lyophilized) 1 Vial |
