SF1 Rabbit Polyclonal Antibody (CAB6424)

(No reviews yet) Write a Review
お見積り依頼
SKU:
CAB6424
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse
Synonyms:
BBP, MBBP, ZFM1, ZNF162, D11S636, ZCCHC25, SF1
Research Use:
Epigenetics and Nuclear Signaling
€0.00
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

SF1 Antibody (CAB6424)

The SF1 Antibody (CAB6424) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Splicing factor 1 is known to be involved in the splicing of precursor mRNA molecules, a crucial step in gene expression that can ultimately have a profound impact on protein diversity and cellular function. By targeting the splicing factor 1 protein, this antibody enables precise detection and analysis of splicing factor 1 in various cell types, making it an essential component in studies related to RNA processing and gene regulation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:SF1 Antibody
SKU:CAB6424
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MATG ANAT PLDF PSKK RKRS RWNQ DTME QKTV IPGM PTVI PPGL TREQ ERAY IVQL QIED LTRK LRTG DLGI PPNP EDRS PSPE PIYN SEGK RLNT REFR TRKK LEEE RHNL ITEM VALN PDFK PPAD YKPP ATRV SDKV MIPQ DEYP EINF VGLL IGPR GNTL KNIE KECN AKIM IRGK GSVK EGKV GRKD GQML PGED EPLH ALVT ANTM ENVK KAVE QIRN ILKQ GIET PEDQ NDLR KMQL RELA RLNG TLRE DDNR ILRP WQSS ETRS ITNT TVCT
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:BBP, MBBP, ZFM1, ZNF162, D11S636, ZCCHC25, SF1
Positive Sample:HepG2
Cellular Localization:Nucleus.
Calculated MW:68kDa
Observed MW:68kDa

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID7536
RRIDAB_2767026
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HepG2 cells, using SF1 Rabbit pAb (CAB6424) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
View AllClose

View AllClose

Related Products