Description
TNFSF10 Antibody (CAB2138)
The TNFSF10 Antibody (CAB2138) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly specific to human samples, making it ideal for a range of research applications.TNFSF10, also known as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), has been implicated in various cancer types and is being studied for its potential therapeutic applications in cancer treatment.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | TNFSF10 Antibody |
| SKU: | CAB2138 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | MAMM EVQG GPSL GQTC VLIV IFTV LLQS LCVA VTYV YFTN ELKQ MQDK YSKS GIAC FLKE DDSY WDPN DEES MNSP CWQV KWQL RQLV RKMI LRTS EETI | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | TL2, APO2L, CD253, TANCR, TRAIL, Apo-2L, TNLG6A, TNFSF10 |
| Positive Sample: | THP-1 treated with LPS |
| Cellular Localization: | Membrane, Single-Pass Type Ii Membrane Protein. |
| Calculated MW: | 33kDa |
| Observed MW: | 28kDa |
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
| Purification Method | Affinity purification |
| Gene ID | 8743 |
| RRID | AB_2764157 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from THP-1 cells, using (CAB2138) at 1:1000 dilution. THP-1 cells were treated with LPS (1 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s. |
