Description
RPL13A Polyclonal Antibody (CAB22023)
The RPL13A Polyclonal Antibody (CAB22023) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbits, is specifically designed to target RPL13A in human samples and is validated for use in Western blot applications. By effectively binding to the RPL13A protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and genetics.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | RPL13A Polyclonal Antibody |
| SKU: | CAB22023 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | MAEV QVLV LDGR GHLL GRLA AIVA KQVL LGRK VVVV RCEG INIS GNFY RNKL KYLA FLRK RMNT NPSR GPYH FRAP SRIF WRTV RGML PHKT KRGQ AALD RLKV FDGI PPPY DKKK RMVV PAAL KVVR LKPT RKFA YLGR LAHE VGWK YQAV TATL EEKR KEKA KIHY RKKK QLMR LRKQ AEKN VEKK IDKY TEVL K | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | L13A, uL13, TSTA1, RPL13A |
| Positive Sample: | HeLa, RD, HCT116, COS-7 |
| Cellular Localization: | Cytoplasm, Cytosol, Cytosolic Ribosome, Focal Adhesion, Nucleolus, Nucleus. |
| Calculated MW: | 24kDa |
| Observed MW: | 24kDa |
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
| Purification Method | Affinity purification |
| Gene ID | 23521 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using RPL13A Rabbit pAb (CAB22023) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s. |
