The Aim2 Polyclonal Antibody (CAB22874) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits, providing high reactivity with human samples and validated for use in Western blot applications. By binding to the AIM2 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential tool for studies in immunology and infectious disease research.The AIM2 protein is essential for sensing cytoplasmic DNA and activating the inflammasome, leading to the production of pro-inflammatory cytokines and the induction of pyroptosis, a form of inflammatory cell death.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
Aim2 Polyclonal Antibody
SKU:
CAB22874
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Gm1313, Ifi210, Aim2
Positive Sample:
Rat spleen
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
40kDa
Observed MW:
39kDa
Enables double-stranded DNA binding activity and identical protein binding activity. Involved in positive regulation of macromolecule metabolic process; pyroptosis; and regulation of defense response. Acts upstream of or within cellular response to interferon-beta. Located in cytoplasm. Part of AIM2 inflammasome complex. Orthologous to human AIM2 (absent in melanoma 2).
Purification Method
Affinity purification
Gene ID
383619
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat spleen, using Aim2 Rabbit pAb (CAB22874) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
