Anti-NCOA4 Antibody (CAB5695)[KO Validated]

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SKU:
CAB5695
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
RFG, ELE1, PTC3, ARA70, NCOA4
Research Use:
Epigenetics and Nuclear Signaling
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Description

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[KO Validated] NCOA4 Antibody (CAB5695)

The [KO Validated] NCOA4 Antibody (CAB5695) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the NCOA4 protein, this antibody enables the detailed analysis and detection of NCOA4 in various cell types, making it an essential component for studies in the fields of metabolism, autophagy, and cancer research.NCOA4, also known as nuclear receptor coactivator 4, plays a crucial role in the maintenance of iron homeostasis by mediating the degradation of ferritin through autophagy.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] NCOA4 Antibody
SKU:CAB5695
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SEWL IRPP YKEG SPKE VPGT EDRA GKQK FKSP MNTS WCSF NTAD WVLP GKKM GNLS QLSS GEDK WLLR KKAQ EVLL NSPL QEEH NFPP DHYG LPAV CDLF ACMQ LKVD KEKW LYRT PLQM
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RFG, ELE1, PTC3, ARA70, NCOA4
Positive Sample:Rat testis
Cellular Localization:Nucleus.
Calculated MW:70kDa
Observed MW:80kDa

This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.

Purification MethodAffinity purification
Gene ID8031
RRIDAB_2766454
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Rat testis using NCOA4 Rabbit pAb (CAB5695) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung tissue using NCOA4 Rabbit pAb (CAB5695) at a dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of K-562 cells using NCOA4 Rabbit pAb(CAB5695) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of 293F cells using NCOA4 Rabbit pAb(CAB5695) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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