Anti-BAZ1B Antibody (CAB9851)[KO Validated]

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SKU:
CAB9851
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
WSTF, WBSCR9, WBSCR10, TF
Research Use:
Epigenetics and Nuclear Signaling
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Description

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[KO Validated] WSTF Antibody (CAB9851)

The [KO Validated] WSTF Antibody (CAB9851) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.BAZ1B, also known as Williams syndrome transcription factor, is involved in DNA repair, transcriptional activation, and chromatin remodeling processes. Dysregulation of BAZ1B has been linked to developmental disorders, cancer, and other diseases.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] WSTF Antibody
SKU:CAB9851
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAPL LGRK PFPL VKPL PGEE PLFT IPHT QEAF RTRE EYEA RLER YSER IWTC KSTG SSQL THKE AWEE EQEV AELL KEEF PAWY EKLV LEMV HHNT ASLE KLVD TAWL EIMT KYAV GEEC DFEV GKEK MLKV KIVK IHPL EKVD EEAT EKKS DGAC DSPS SDKE NSSQ IAQD HQKK ETVV KEDE GRRE SIND RARR SPRK LPTS LKKG ERKW APPK FLPH KYDV KLQN EDKI ISNV PADS LIRT ERPP NKEI VRYF IRHN
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:WSTF, WBSCR9, WBSCR10, TF
Positive Sample:SW480, BT-474, SKOV3, HeLa, HepG2, Mouse thymus
Cellular Localization:Nucleus.
Calculated MW:171kDa
Observed MW:190kDa

This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.

Purification MethodAffinity purification
Gene ID9031
RRIDAB_2863781
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] WSTF Rabbit pAb (CAB9851) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageWestern blot analysis of lysates from wild type (WT) and WSTF knockout (KO) HeLa cells, using [KO Validated] WSTF Rabbit pAb (CAB9851) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] WSTF Rabbit pAb (CAB9851) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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