The Mouse TRAIL (TNFSF10) ELISA Kit is a powerful tool for the precise measurement of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) levels in mouse serum, plasma, and cell culture supernatants. This kit is designed with optimal sensitivity and specificity for accurate and reproducible results, making it perfect for various research applications.TRAIL is a key protein involved in apoptosis, playing a crucial role in immune response and cell death regulation. Its dysregulation has been linked to cancer, autoimmune diseases, and inflammatory conditions, making it a valuable biomarker for investigating these diseases and potential therapeutic interventions. Get reliable and insightful data with the Mouse TRAIL (TNFSF10) ELISA Kit to advance your research efforts and uncover new insights into TRAIL biology and its role in disease development. Visit assaygenie.com/mouse-trail-tnfsf10-tumor-necrosis-factor-related-apoptosis-inducing-ligand-elisa-kit-moes01496/ for more information and to order your kit today.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
87-101
85-99
96-110
Average (%)
92
92
101
1:4
Range (%)
91-101
86-100
87-100
Average (%)
96
92
92
1:8
Range (%)
86-99
82-93
88-101
Average (%)
93
88
93
1:16
Range (%)
86-100
85-97
86-101
Average (%)
93
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
93-107
99
Cell Culture Media (n=8)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.17
107.54
473.36
49.96
105.95
443.33
Standard deviation
3.18
6.03
19.79
3.12
7.86
33.12
C V (%)
6.61
5.61
4.18
6.24
7.42
7.47
Uniport ID:
P50592
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Mouse TRAIL/TNFSF10 and analogues was observed
