The [KO Validated] PNP Antibody (CAB19885) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has been rigorously validated for use in various applications, including Western blotting and immunohistochemistry.PNP, also known as purine nucleoside phosphorylase, is a key enzyme involved in the breakdown of purine nucleosides, which are essential for DNA and RNA synthesis. Dysregulation of PNP activity has been linked to various diseases, including immune deficiencies and autoimmune disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] PNP Antibody
SKU:
CAB19885
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NP, PUNP, PRO1837
Positive Sample:
293T
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
32kDa
Observed MW:
30kDa
This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
Purification Method
Affinity purification
Gene ID
4860
RRID
AB_2862796
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PNP Rabbit pAb (CAB19885) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates using PNP Rabbit pAb (CAB19885) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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