[KO Validated] alpha-Catenin Rabbit Monoclonal Antibody (CAB19004)

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SKU:
CAB19004
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
MDBS2, MDPT2, CAP102, in
Research Use:
Cell Biology
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Description

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[KO Validated] alpha-Catenin Monoclonal Antibody (CAB19004)

The [KO Validated] alpha-Catenin Monoclonal Antibody (CAB19004) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, generated in rabbits, demonstrates strong reactivity with knockout (KO) samples and has been rigorously validated for various applications, including immunofluorescence and immunoprecipitation.Alpha catenin plays a crucial role in maintaining tissue integrity and regulating cell migration, making it a central player in processes like embryonic development and wound healing.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] alpha-Catenin Monoclonal Antibody
SKU:CAB19004
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0348
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MTAV HAGN INFK WDPK SLEI RTLA VERL LEPL VTQV TTLV NTNS KGPS NKKR GRSK KAHV LAAS VEQA TENF LEKG DKIA KESQ FLKE ELVA AVED VRKQ
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MDBS2, MDPT2, CAP102, in
Positive Sample:HCT116, NIH/3T3, Mouse lung, Rat heart, 293T（WT）
Cellular Localization:Cell Junction, Cell Junction, Cell Membrane, Cytoplasm, Cytoplasmic Side, Peripheral Membrane Protein, Adherens Junction, Cytoskeleton.
Calculated MW:100kDa
Observed MW:100kDa

This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy.

Purification MethodAffinity purification
Gene ID1495
RRIDAB_2862496
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and α-Catenin knockout (KO) 293T cells, using [KO Validated] α-Catenin Rabbit mAb (CAB19004) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of various lysates using [KO Validated] α-Catenin Rabbit mAb (CAB19004) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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