[KO Validated] DNMT1 Rabbit Monoclonal Antibody (CAB19679)

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SKU:
CAB19679
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN, m.HsaI, [KD Validated] DNMT1
Research Use:
Epigenetics and Nuclear Signaling
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Description

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[KO Validated] DNMT1 Monoclonal Antibody (CAB19679)

The [KO Validated] DNMT1 Monoclonal Antibody (CAB19679) is a high-quality antibody developed for reliable detection and analysis of target proteins. DNMT1 is essential for maintaining DNA methylation patterns during cell division, and dysregulation of this enzyme has been implicated in numerous diseases, including cancer and neurological disorders.This antibody, specifically raised in rabbits, has been rigorously tested and validated for knockout (KO) studies, ensuring high specificity and sensitivity in detecting DNMT1 protein levels. It is suitable for use in techniques such as Western blotting, immunofluorescence, and immunoprecipitation, allowing for detailed analysis of DNMT1 expression and localization in different cell types and experimental conditions.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] DNMT1 Monoclonal Antibody
SKU:CAB19679
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC51348
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MPAR TAPA RVPT LAVP AISL PDDV RRRL KDLE RDSL TEKE CVKE KLNL LHEF LQTE IKNQ LCDL ETKL RKEE LSEE GYLA KVKS LLNK DLSL ENGA HAYN
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:1000 - 1:4000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN, m.HsaI, [KD Validated] DNMT1
Positive Sample:Jurkat, HeLa, 293T
Cellular Localization:Nucleus.
Calculated MW:183kDa
Observed MW:200kDa

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID1786
RRIDAB_2862730
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KD Validated] DNMT1 Rabbit mAb (CAB19679) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 30 s.
Product ImageWestern blot analysis of lysates from wild type (WT) and DNMT1 knockdown (KD) 293T cells, using [KD Validated] DNMT1 Rabbit mAb (CAB19679) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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