The HRP-conjugated Mouse anti His-Tag mAb (CABE028) is a high-quality antibody developed for reliable detection and analysis of target proteins. The His-tag consists of consecutive histidine residues (typically 6–10) genetically inserted into recombinant protein sequences, enabling efficient affinity purification via coordination of divalent metal ions on IMAC resins. His-tagged proteins can be detected using anti-His antibodies in western blotting, ELISA, and other immunoassay formats.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
HRP-conjugated Mouse anti His-Tag mAb
SKU:
CABE028
Size:
50μL, 100μL, 200μL
Reactivity:
Species independent
Clone Number:
AMC0484
Conjugate:
HRP
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:10000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
His, His tag, His-tag
Positive Sample:
293T
Observed MW:
RefertoFigures
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
Purification Method
Affinity purification
RRID
AB_2769867
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein,using HRP-conjugated Mouse anti His-Tag mAb (CABE028) at 1:20000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
