The HRP-conjugated Mouse anti HA-Tag mAb (CABE025) is a high-quality antibody developed for reliable detection and analysis of target proteins. The HA-tag (YPYDVPDYA) is a short epitope derived from influenza hemagglutinin genetically incorporated into recombinant proteins to provide a specific immunological handle for detection and purification. It is among the most widely used epitope tags due to the high affinity and broad availability of validated anti-HA antibodies for western blotting, immunofluorescence, and immunoprecipitation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
HRP-conjugated Mouse anti HA-Tag mAb
SKU:
CABE025
Size:
100μL, 200μL
Reactivity:
Species independent
Clone Number:
AMC0557
Conjugate:
HRP
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:10000 - 1:20000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
RRID
AB_2769866
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of normal 293T cells, 293T transfected with SCRN1 Protein , using HRP-conjugated Mouse anti HA-Tag mAb (CABE025) at 1:5000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with CD2 Protein and HA-N Protein ,293F transfected with CD95-N Protein using HRP-conjugated Mouse anti HA-Tag mAb (CABE025) at 1:5000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
