Description
GenieLadder DNA Loading Buffer (6X)
GenieLadder DNA Loading Buffer is a ready-to-use 6x concentrated gel-loading buffer for preparing DNA samples ahead of agarose gel electrophoresis. It combines a high-density agent with a bromophenol blue tracking dye so samples sink cleanly into wells and migration can be followed by eye.
The formulation has been optimised for convenient handling and stability, and can be stored at room temperature for extended periods without loss of performance.
| Product Name: | GenieLadder DNA Loading Buffer (6X) |
| Brand: | GenieLadder |
| Product type: | Gel-loading buffer |
| Concentration: | 6x (dilute to 1x in final sample) |
| Tracking dye: | Bromophenol blue |
| Storage: | Stable at room temperature; 4 C also suitable. |
| Shipping: | Ships at ambient temperature. |
| Available sizes: | 1 ml |
- 6x concentrate - simply mix one part buffer with five parts sample.
- High density ensures samples settle neatly into wells for clean, straight bands.
- Bromophenol blue tracking dye stays clearly visible even after dilution to 1x.
- Room-temperature stable for convenient benchtop storage.
- Preparing DNA samples for agarose gel electrophoresis
- Loading PCR products, digests and plasmid preps
- General nucleic-acid electrophoresis workflows
| Step | Procedure |
| 1 | Add 1 volume of 6x loading buffer to every 5 volumes of DNA sample (e.g. 2 ul buffer + 10 ul sample). |
| 2 | Mix gently by pipetting. |
| 3 | Load the mixture into the wells of your agarose gel. |
| 4 | Run electrophoresis until the bromophenol blue front has migrated the required distance, then visualise. |
What is the working concentration?
The buffer is a 6x concentrate. Combine one part buffer with five parts sample so the final loading concentration is 1x.
Which dye front does it use?
It contains bromophenol blue, which migrates as a visible band so you can monitor the run and stop before samples run off the gel.
How should I store it?
It is stable at room temperature, which makes it convenient to keep on the bench; storage at 4 C is also fine.
