Description
GenieDetect Human Telomere Length qPCR Assay Kit (Relative)
The GenieDetect Human Telomere Length qPCR Assay Kit (Relative) is a relative-quantification qPCR assay for measuring average telomere length in human genomic DNA samples. Telomeres are tandem TTAGGG repeats that cap chromosome ends, protecting them from degradation and fusion; their length shortens with cell division and is widely studied as a marker of ageing, DNA repair and disease.
The kit determines relative telomere length as the ratio (T/S) of the telomere repeat signal (T) to a single-copy reference gene (S). The single-copy primers specifically amplify a 78 bp region on human chromosome 11. Every primer set is validated for amplification efficiency (E greater than 98%, R2 greater than 0.99), melt-curve profile and gel electrophoresis to ensure specific, reproducible quantification.
| Product Name: | GenieDetect Human Telomere Length qPCR Assay Kit (Relative) |
| Brand: | GenieDetect |
| Product type: | Relative telomere-length qPCR assay kit |
| Species: | Human |
| Method: | Relative quantification, telomere-to-single-copy-gene (T/S) ratio |
| Reference gene amplicon: | 78 bp region on human chromosome 11 |
| Primer validation: | E greater than 98%, R2 greater than 0.99; melt-curve and gel verified |
| Instrument compatibility: | All common real-time PCR systems (standalone ROX supplied) |
| Storage: | Store at -20 C, protected from light. Avoid repeated freeze-thaw. |
| Shipping: | Ships on dry ice / blue ice. |
| Available sizes: | 100 reactions, 500 reactions |
- Relative telomere length by the established T/S ratio method.
- Validated primer sets: efficiency E greater than 98%, R2 greater than 0.99, confirmed by melt-curve and gel analysis.
- Optimised ready-to-use master mix for fast, reproducible qPCR.
- Balanced K+/NH4+ chemistry with separately packaged ROX reference dye for compatibility with all common qPCR instruments.
- Relative telomere-length measurement in research samples
- Ageing, senescence and longevity research
- Studies of DNA damage/repair, apoptosis and tumour biology
- Comparative T/S analysis across human sample cohorts
| Step | Procedure |
| 1 | Purify high-quality genomic DNA from your human samples and normalise input concentrations. |
| 2 | Set up parallel qPCR reactions for the telomere (T) and single-copy reference (S) targets using the supplied master mix and primers. |
| 3 | Run the recommended qPCR programme, including standard/reference samples for relative quantification. |
| 4 | Calculate the T/S ratio for each sample to derive relative telomere length. |
Note: Accurate, equal DNA input across samples is critical for reliable T/S ratios. Run reactions in replicate and include a calibrator sample for cross-plate comparison.
What does this kit actually measure?
It measures relative average telomere length as the T/S ratio - the telomere repeat signal divided by a single-copy reference-gene signal - rather than an absolute length in kilobases.
Which instruments can I use?
The chemistry is compatible with all common real-time PCR instruments; ROX reference dye is supplied separately so you can add it only if your instrument requires it.
How were the primers validated?
Each primer set is confirmed for amplification efficiency (E greater than 98%, R2 greater than 0.99) alongside melt-curve analysis and gel electrophoresis to rule out non-specific products.
What sample material do I need?
High-quality human genomic DNA, with input amounts normalised across all samples for meaningful comparison.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
