Description
GenieDetect Nipah Virus Detection Kit (TaqMan Probe)
The GenieDetect Nipah Virus Detection Kit (TaqMan Probe) is a research-use probe-based real-time PCR assay for detecting Nipah virus (NiV) nucleic acid. The TaqMan probe format adds a sequence-specific detection layer on top of primer specificity, supporting confident identification of NiV targets in research and surveillance samples.
The kit includes primers and a probe designed against the conserved NiV P (phosphoprotein) region, HotStart Taq DNA polymerase and an optimised qPCR buffer system for efficient amplification of low-concentration templates. A positive control is supplied for assay validation and false-negative differentiation.
| Product Name: | GenieDetect Nipah Virus Detection Kit (TaqMan Probe) |
| Brand: | GenieDetect |
| Product type: | TaqMan probe-based real-time PCR kit |
| Target: | Conserved region of the NiV P (phosphoprotein) gene |
| Key components: | NiV-specific primers and probe, HotStart Taq DNA polymerase, optimised qPCR buffer, positive control |
| Controls: | Positive control included |
| Storage: | Store at -20 C, protected from light. Avoid repeated freeze-thaw. |
| Shipping: | Ships on dry ice / blue ice. |
| Available sizes: | 50 reactions, 100 reactions |
- Sequence-specific TaqMan probe for high-confidence detection.
- Primers and probe target the conserved NiV P gene region.
- Ready-to-use; only the template needs to be added.
- No cross-reactivity to other common pathogens tested; positive control included.
- Research detection of Nipah virus nucleic acid
- Surveillance and epidemiological research studies
- Probe-based qPCR assay development in molecular virology
| Step | Procedure |
| 1 | Extract nucleic acid from the sample using a suitable method. |
| 2 | Assemble the probe-based reaction mix per the kit insert and add template. |
| 3 | Include the supplied positive control and a no-template control in each run. |
| 4 | Run the qPCR programme and interpret probe fluorescence according to the insert. |
Note: Refer to the product insert for exact reaction set-up, cycling conditions and result interpretation. Always include the recommended controls.
How does the TaqMan format help?
The probe hybridises to a specific internal sequence between the primers, adding a second layer of specificity beyond the primers alone for high-confidence calls.
What is included in the kit?
NiV-specific primers and probe, HotStart Taq DNA polymerase, an optimised qPCR buffer system and a positive control.
Which instruments are compatible?
Any standard real-time PCR instrument able to read the probe's reporter dye channel is suitable.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
