The CXCL10 Antibody (CAB19138) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly sensitive and suitable for use in various experimental techniques, including Western blotting.CXCL10, also known as interferon-gamma-induced protein 10 (IP-10), is a key player in the recruitment and activation of immune cells at sites of infection or inflammation. Its upregulation is associated with various inflammatory conditions, making it a crucial target for research in immunology, infectious diseases, and autoimmune disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CXCL10 Antibody
SKU:
CAB19138
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
VPLS RTVR CTCI SISN QPVN PRSL EKLE IIPA SQFC PRVE IIAT MKKK GEKR CLNP ESKA IKNL LKAV SKER SKRS P
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. This gene may also be a key regulator of the 'cytokine storm' immune response to SARS-CoV-2 infection.
Purification Method
Affinity purification
Gene ID
3627
RRID
AB_2862631
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from THP-1 cells, using CXCL10/IP-10 Rabbit pAb (CAB19138) at 1:3000 dilution. THP-1 cells were treated with LPS for 6 hours,Brefeldin A for 3 hours and IFNγ (50ng/ml) for 8 hours of stimulation. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of NIH/3T3 cells using CXCL10/IP-10 Rabbit pAb (CAB19138) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
