The USP53 Polyclonal Antibody (CAB24873) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and sensitivity in detecting USP53 in human samples, making it suitable for Western blot applications. By targeting the USP53 protein, this antibody allows for the precise analysis of USP53 expression and function in different cell types, facilitating research in fields such as cancer biology and DNA damage response pathways.USP53, also referred to as ubiquitin-specific protease 53, plays a crucial role in maintaining genome stability and ensuring proper cell division.
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
USP53 Polyclonal Antibody
SKU:
CAB24873
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
USP53, ubiquitin specific peptidase 53
Positive Sample:
Mouse placenta
Cellular Localization:
Cell Junction, Tight Junction.
Calculated MW:
120kDa
Observed MW:
120kDa
Purification Method
Affinity purification
Gene ID
54532
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse placenta using USP53 Rabbit pAb (CAB24873) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of paraffin-embedded Human liver cancer using USP53 Rabbit pAb(CAB24873) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
