The USP10 Antibody (CAB7505) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the USP10 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal tool for immunology and cancer research studies.USP10 is known for its involvement in regulating the stability and activity of numerous proteins through the removal of ubiquitin molecules.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
USP10 Antibody
SKU:
CAB7505
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UBPO, USP10
Positive Sample:
HepG2, Mouse brain
Cellular Localization:
Cytoplasm, Early Endosome, Nucleus.
Calculated MW:
87kDa
Observed MW:
110kDa
Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene.
Purification Method
Affinity purification
Gene ID
9100
RRID
AB_2768036
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using USP10 Rabbit pAb (CAB7505) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of A549 cells using USP10 Rabbit pAb (CAB7505). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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