Type I / II Interferon Antibody Sampler Pack

Type I / II Interferon Antibody Sampler Pack

The Type I / II Interferon Antibody Sampler Pack from Assay Genie brings together 8 rigorously validated antibodies spanning the key nodes of this pathway, supplied in a convenient 20µL trial size. It lets researchers screen an entire signalling axis in a single, cost-effective pack — validated for applications including ChIP-seq, ELISA, IF/ICC, IHC-P, IP, WB — before committing to full-size vials. Each antibody is also available individually in a full 100µL size.

Interferons are a family of cytokines that constitute a principal arm of antiviral defense and immunoregulation, coordinating cell-intrinsic resistance to infection and shaping adaptive immunity. The response begins with cytosolic sensing of viral nucleic acids. The RNA helicase RIG-I/DDX58 detects short, blunt-ended, and 5'-triphosphorylated viral RNA, undergoing a conformational change that nucleates a signaling cascade through the mitochondrial adaptor MAVS, ultimately activating interferon regulatory factors. IRF7 is the master amplifier of type I interferon production, driving robust expression of IFN-alpha and IFN-beta, particularly in a feed-forward loop that escalates the antiviral response. Secreted type I interferons act in autocrine and paracrine fashion through their receptor to activate the JAK-STAT pathway, assembling the trimeric ISGF3 complex from STAT1, STAT2, and the DNA-binding partner IRF9, which binds interferon-stimulated response elements to induce hundreds of interferon-stimulated genes (ISGs). Type II interferon, IFN-gamma, signals predominantly through STAT1 homodimers to enhance macrophage activation and antigen presentation. The induced ISGs constitute the antiviral effector arsenal: ISG15 is a ubiquitin-like modifier that conjugates to viral and host proteins to restrict replication, IFITM3 blocks viral entry and fusion at endosomal membranes, and the oligoadenylate synthetase OAS3 senses double-stranded RNA and activates RNase L to degrade viral and cellular RNA. While essential for host defense, sustained or aberrant interferon signaling drives autoimmune interferonopathies and contributes to inflammatory disease. Dissecting sensing, signal transduction, and effector output requires reagents spanning each layer. This sampler pack brings together validated antibodies against RIG-I/DDX58, IRF7, IRF9, STAT1, STAT2, ISG15, IFITM3, and OAS3 for studying type I and type II interferon responses.