Description
DNA topoisomerase II alpha (TOP2A) Antibody (CAB16440)
The DNA topoisomerase II alpha (TOP2A) Antibody (CAB16440) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in Western blot applications, providing reliable detection and analysis of the TOP2A protein in a variety of cell types.TOP2A is essential for DNA unwinding during replication and is a target for a number of anti-cancer drugs.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | DNA topoisomerase II alpha (TOP2A) Antibody |
| SKU: | CAB16440 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | EEEN EESD NEKE TEKS DSVT DSGP TFNY LLDM PLWY LTKE KKDE LCRL RNEK EQEL DTLK RKSP SDLW KEDL ATFI EELE AVEA KEKQ DEQV GLPG KGGK A | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | TOP2, TP2A, TOPIIA, TOP2alpha, DNA topoisomerase II alpha (TOP2A) |
| Positive Sample: | HeLa, 293T |
| Cellular Localization: | Cytoplasm, Nucleus, Nucleoplasm. |
| Calculated MW: | 174kDa |
| Observed MW: | 190kDa |
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
| Purification Method | Affinity purification |
| Gene ID | 7153 |
| RRID | AB_2772684 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using DNA topoisomerase II alpha (TOP2A) Rabbit pAb (CAB16440) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
