The TNNI3 Antibody (CAB0152) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it ideal for use in Western blot applications. By binding specifically to the TNNT3 protein, researchers can accurately detect and analyze its expression in various cell types, aiding in the study of heart function and cardiovascular diseases.TNNT3 is a crucial component of the troponin complex, which regulates muscle contraction in the heart. Mutations in TNNT3 have been linked to various heart conditions, making it a potential target for therapeutic intervention.
This antibody is validated for use in WB, IP, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
TNNI3 Antibody
SKU:
CAB0152
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CMH7, RCM1, cTnI, CMD2A, TNNC1, CMD1FF, Cardiac Troponin I (TNNI3)
Positive Sample:
Mouse heart, Rat lung
Cellular Localization:
Cardiac Myofibril, Cytosol, Sarcomere.
Calculated MW:
24kDa
Observed MW:
28kDa
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19.
Purification Method
Affinity purification
Gene ID
7137
RRID
AB_2756984
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse heart, using Cardiac Troponin I (TNNI3) Rabbit pAb (CAB0152) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of lysates from Rat lung, using Cardiac Troponin I (TNNI3) Rabbit pAb (CAB0152) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunoprecipitation analysis of 600 μg extracts of Mouse heart cells using 3 μg Cardiac Troponin I (TNNI3) antibody (CAB0152). Western blot was performed from the immunoprecipitate using Cardiac Troponin I (TNNI3) antibody (CAB0152) at a dilution of 1:1000.
