The DMT1 Antibody (CAB10231) is a high-quality antibody developed for reliable detection and analysis of target proteins. This protein is involved in the transport of divalent metals such as iron and manganese across cell membranes, making it essential for maintaining metal homeostasis in various tissues.Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the SLC11A2 protein, researchers can effectively detect and analyze its expression in different cell types, facilitating studies in fields such as metabolism, neurobiology, and iron-related disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DMT1 Antibody
SKU:
CAB10231
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4891
RRID
AB_2757756
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DMT1/SLC11A2 Rabbit pAb (CAB10231) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat stomach using DMT1/SLC11A2 Rabbit pAb (CAB10231) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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