Sheep Estrogen ELISA Kit
- SKU:
- SHFI00063
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Sensitivity:
- 9.375pg/ml
- Range:
- 15.625-1000pg/ml
- ELISA Type:
- Competitive ELISA, Coated with Antigen
- Synonyms:
- ESRRG, Estrogen-related receptor gamma, ERR3, NR3B3, ERR gamma-2, ERRG2, ERRgamma, Estrogen receptor-related protein 3, NR3B3, Nuclear receptor subfamily 3 group B member 3, KIAA0832
- Reactivity:
- Sheep
Description
| Information | Description |
| Product name | Sheep Estrogen ELISA Kit |
| Catalogue No. | SHFI00063 |
| Size | 96T |
| Sensitivity | 9.375pg/ml |
| Detection method | Competitive ELISA, Double Antibody |
| Range | 15.625-1000pg/ml |
| Application | For quantitative detection of Estrogen in serum, plasma, tissue homogenates and other biological fluids. |
| Recovery | Matrices listed below were spiked with certain level of Estrogen and the recovery rates were calculated by comparing the measured value to the expected amount of Estrogen in samples. |
| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estrogen and their serial dilutions. The results were demonstrated by percentage of calculated concentration to the expectation. |
| CV (%) | Intra-Assay: CV<8% |
| Storage | 2-8°C for 6 months |
Sheep Estrogen ELISA Kit Protocol
The below protocol is a sample protocol for Sheep Progesterone ELISA Kit using a biotinylated detection antibody and streptavidin-HRP. Competitive ELISAs allow for the detection and quantification of an analyte in a sample by using known analyte concentrations as standards and plotting absorbance of known concentrations vs known standard concentrations. This allows the researcher to calculate the amount of Sheep Progesterone present in their sample.
Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37 °C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.
Competitive Protocol
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