The SEPHS1 Antibody (CAB13403) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for Sephs1 and is validated for use in various applications, including Western blotting. By binding to Sephs1, this antibody enables detection and analysis of the protein in a variety of cell types, making it ideal for studies in biochemistry and cell biology.Sephs1 is a crucial component of the redox regulatory system in cells, where it helps maintain the balance of reactive oxygen species and protect against oxidative stress.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SEPHS1 Antibody
SKU:
CAB13403
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPS, SELD, SPS1, S1
Positive Sample:
293T, BT-474, SW620, HepG2, Rat kidney
Cellular Localization:
Cell Membrane, Cytoplasm, Nucleus Membrane.
Calculated MW:
43kDa
Observed MW:
40kDa
This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.
Purification Method
Affinity purification
Gene ID
22929
RRID
AB_2760264
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SEPHS1 Rabbit pAb (CAB13403) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from wild type(WT) and SEPHS1 knockout (KO) 293T(KO) cells, using [KO Validated] SEPHS1 Rabbit pAb (CAB13403) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human leiomyoma of uterus using SEPHS1 Rabbit pAb (CAB13403) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using SEPHS1 Rabbit pAb (CAB13403). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
