The [KO Validated] SEC61B Antibody (CAB15788) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications.The SEC61B antibody binds specifically to the SEC61B protein, enabling precise detection and analysis in a variety of cell types. This makes it an invaluable asset for studies related to protein trafficking, protein folding, and quality control in the endoplasmic reticulum. Researchers in the fields of cell biology, molecular biology, and biochemistry will find this antibody indispensable for their work.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] SEC61B Antibody
SKU:
CAB15788
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.
Purification Method
Affinity purification
Gene ID
10952
RRID
AB_2763208
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and SEC61B knockout (KO) 293T cells, using [KO Validated] SEC61B Rabbit pAb (CAB15788) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of L929 cells using [KO Validated] SEC61B Rabbit pAb (CAB15788) at dilution of 1:100. Blue: DAPI for nuclear staining.
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