The RNF149 Antibody (CAB15627) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through immunization of rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the RNF149 protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for studies in immunology and cancer research.RNF149, a RING finger protein, plays a crucial role in the maintenance of cellular homeostasis by mediating protein ubiquitination and degradation.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RNF149 Antibody
SKU:
CAB15627
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DNAPTP2, RNF149
Positive Sample:
Mouse stomach,
Cellular Localization:
Cytoplasm.
Calculated MW:
43kDa
Observed MW:
43kDa
Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in ubiquitin-dependent protein catabolic process. Predicted to act upstream of or within cellular response to xenobiotic stimulus; negative regulation of MAPK cascade; and regulation of protein stability. Located in membrane.
Purification Method
Affinity purification
Gene ID
284996
RRID
AB_2763034
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse stomach using RNF149 Rabbit pAb (CAB15627) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of NIH/3T3 cells using RNF149 Rabbit pAb (CAB15627) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
