Description
Recombinant Human TNFSF14/LIGHT/HVEM-L/CD258 Protein
The Recombinant Human TNFSF14/LIGHT/HVEM-L/CD258 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating TNFSF14/LIGHT/HVEM-L/CD258 function and its implications in health and disease.
This product (SKU: RPCB0959) is produced using HEK293 cells and features a N-his tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 19.05 kDa with an observed molecular weight of 20-25 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human TNFSF14/LIGHT/HVEM-L/CD258 Protein |
| SKU: | RPCB0959 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | TNFSF14, CD258, HVEML, LIGHT, LTg |
| Tag: | N-his |
| Expression Host: | HEK293 cells |
| Calculated MW: | 19.05 kDa |
| Observed MW: | 20-25 kDa |
| Gene ID: | 8740 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human TNFSF14/LIGHT/HVEM-L/CD258 Protein (RPCB0959), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | Please contact us for more information. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF14 at 2 μg/mL (100 μL/well) can bind Mouse HVEM with a linear range of 0.02-0.89 μg/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 / LIGHT / CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin β receptor (LTβR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 / LIGHT / CD258 also binds to a soluble non-signaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14 / LIGHT / CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4+and CD8+T cells. In addition, TNFSF14 / LIGHT / CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 / LIGHT / CD258 is being actively investigated as a possible basis for cancer treatment.
 | Recombinant Human TNFSF14/LIGHT/HVEM-L/CD258 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized Recombinant Human TNFSF14 at 2 μg/mL (100 μL/well) can bind Mouse HVEM with a linear range of 0.02-0.89 μg/mL. |
