Description
Recombinant Human Serpin F2/Alpha-2-antiplasmin Protein
The Recombinant Human Serpin F2/Alpha-2-antiplasmin Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Serpin F2/Alpha-2-antiplasmin function and its implications in health and disease.
This product (SKU: RPCB0237) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 52.56 kDa with an observed molecular weight of 60-80 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human Serpin F2/Alpha-2-antiplasmin Protein |
| SKU: | RPCB0237 |
| Size: | 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | SERPINF2, A2AP, AAP, ALPHA-2-PI, API, PLI |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 52.56 kDa |
| Observed MW: | 60-80 kDa |
| Gene ID: | 5345 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Serpin F2/Alpha-2-antiplasmin Protein (RPCB0237), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 90 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <0.23 nM. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots. In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta also express this protein at a moderate level.SerpinF2 is one of the inhibitors of fibrinolysis. It is a specific plasmin inhibitor, which degrades fibrin and various other proteins. Consequently, the protein plays the dominant role in regulating the blood clotting pathway. Mutations in SerpinF2 gene lead to alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.
 | Recombinant Human Serpin F2/Alpha-2-antiplasmin Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Recombinant human SERPINF2 inhibits trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <0.23nM. |
