The Recombinant Human Oncostatin-M/OSM Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Oncostatin-M/OSM function and its implications in health and disease.
This product (SKU: RPCB0136) is produced using E. coli and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 22.15 kDa with an observed molecular weight of 24 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human Oncostatin-M/OSM Protein (RPCB0136), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, 5% glycerol, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED 50 for this effect is typically 0.8-3.2 ng/mL, corresponding to a specific activity of 3.12×10 5 -1.25×10 6 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
This protein is a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family. The preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells.
Recombinant Human Oncostatin-M/OSM Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human OSM promotes the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.8-3.2 ng/mL, corresponding to a specific activity of 3.12×105-1.25×106units/mg.
