The Recombinant Human Flt3 ligand/Flt3L Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Flt3 ligand/Flt3L function and its implications in health and disease.
This product (SKU: RPCB0292) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 18.88 kDa with an observed molecular weight of 20-37 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human Flt3 ligand/Flt3L Protein (RPCB0292), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
1、Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Flt3 ligand at 2 μg/mL (100 μL/well) can bind Recombinant Human Flt-3 with a linear range of 0.128-3.97 ng/mL.2、Measured in a cell proliferation assay using Human OCI-AML5 cells. The ED 50 for this effect is 0.09-0.36 ng/mL, corresponding to a specific activity of 2.78×10 6 ~1.11×10 7 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
FLT3LG, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors.FLT3LG is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Alternate splicing of human FLT3LG also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble FLT3LG signal through the tyrosine kinase receptor Flt-3/Flk-2. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation.
Recombinant Human Flt3 ligand/Flt3L Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Recombinant Human Flt3 ligand/Flt3L Protein is greater than 95% as determined by SEC-HPLC.
Measured in a cell proliferation assay using Human OCI-AML5 cells. The ED50 for this effect is 0.09-0.36 ng/mL, corresponding to a specific activity of 2.78×106~1.11×107 units/mg.
