Recombinant Human Apolipoprotein A-I/APOA1 Protein
The Recombinant Human Apolipoprotein A-I/APOA1 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Apolipoprotein A-I/APOA1 function and its implications in health and disease.
This product (SKU: RPCB0243) is produced using E. coli and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 28.28 kDa with an observed molecular weight of 25-30 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Apolipoprotein A-I/APOA1 Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Apolipoprotein A-I/APOA1 Protein (RPCB0243), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA.Immobilized Mouse CD117 (RPCB1586) at 5 μg/mL (100 μL/well) can bind Mouse SCF (RPCB0809) with a linear range of 0.01-2 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
This protein is the major protein component of high density lipoprotein (HDL) in plasma. The preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters.
Recombinant Human Apolipoprotein A-I/APOA1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured by its binding ability in a functional ELISA. Immobilized Human A-I/APOA1 (Cat: RPCB0766) at 5μg/mL (100 μL/well) can bind Human MSR1/CD204 (Cat: RPCB0243) with a linear range of 1-49.4ng/mL.
