Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
The Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is a precise and efficient assay designed to quantitatively measure the levels of TIMP-1 in various biological samples. TIMP-1 is a critical regulator of matrix metalloproteinases and plays a key role in tissue remodeling, cell proliferation, and maintaining tissue homeostasis. Abnormal TIMP-1 levels are associated with pathologies such as cancer, fibrosis, and cardiovascular diseases. Accurate quantification of TIMP-1 is essential for investigating its involvement in physiological and pathological processes, providing valuable insights into disease mechanisms and potential therapeutic strategies. This ELISA kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. With stringent quality control measures in place, this kit delivers robust performance and ease of use, making it an excellent tool for cutting-edge research applications.
Product Name:
Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
SKU:
AEES00447
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
