Rat CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit
The Rat CTX-II (Cross-linked C-telopeptide of Type II Collagen) ELISA Kit is specifically designed for the accurate measurement of CTX-II levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.CTX-II is a key biomarker associated with cartilage degradation and osteoarthritis, making it a valuable tool for studying joint health and potential treatments. Monitoring CTX-II levels can provide important insights into disease progression and therapeutic efficacy, making this ELISA kit essential for researchers investigating musculoskeletal disorders.
Product Name:
Rat CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit
SKU:
RTES01165
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
