Rat ACE (Angiotensin I Converting Enzyme) ELISA Kit
The Rat ACE (Angiotensin I-Converting Enzyme) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of ACE levels in rat serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it ideal for a variety of research applications.ACE is a key enzyme involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance. Dysregulation of ACE activity is associated with various cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.With its high sensitivity and accuracy, the Rat ACE ELISA Kit from Assay Genie provides researchers with a powerful tool for studying ACE levels in rat samples, advancing our understanding of cardiovascular health and disease.
Product Name:
Rat ACE (Angiotensin I Converting Enzyme) ELISA Kit
SKU:
RTES01029
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
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