The RAP1B Polyclonal Antibody (CAB24472) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human and mouse samples and is validated for use in Western blot applications.By binding specifically to the RAP1B protein, this antibody allows for the detection and analysis of RAP1B levels in different cell types, making it a valuable asset for studies in cell biology, cancer research, and signal transduction pathways.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RAP1B Polyclonal Antibody
SKU:
CAB24472
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VYSI TAQS TFND LQDL REQI LRVK DTDD VPMI LVGN KCDL EDER VVGK EQGQ NLAR QWNN CAFL ESSA KSKI NVNE IFYD LVRQ INRK TPVP GKAR KKSS C
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:100 - 1:500
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAP1B, K-REV, RAL1B, ras-related protein Rap-1b
Positive Sample:
Rat brain
Cellular Localization:
Cell Junction, Cell Membrane, Cytoplasm, Cytosol.
Calculated MW:
15kDa/16kDa/18kDa/20kDa
Observed MW:
23kDa
This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9.
Purification Method
Affinity purification
Gene ID
5908
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat brain, using RAP1B Rabbit pAb (CAB24472) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
