Protein A ELISA Kit (GXEL00387)

Protein A ELISA Kit

Assay Genie's Protein A ELISA Kit (SKU: GXEL00387) provides researchers with a reliable quantitative ELISA for the quantitative measurement of Protein A in Plasma, Serum. Offering a measurement range of 0.078-5 ng/mL and a sensitivity of 20 pg/mL, the kit is optimised for precision and ease of use in the research laboratory.

<p>Protein A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Protein A. Samples containing Protein A are first diluted in the Sample Diluent provided with the kits. The Denaturing Buffer is then added and mixed to dissociate the Protein A from the product antibody. The samples are then reacted in microtiter strips coated with a monoclonal anti-Protein A capture antibody. A second anti-Protein A biotinylated monoclonal antibody is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A: biotin labeled antibody. After a wash step to remove any unbound reactants, Streptavidin labeled HRP is added to each well. After a short incubation period, the strips are then reacted with TMB substrate. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (OD) to the concentration of standards. The Protein A concentration in each sample is interpolated from this standard curve.</p>

Complete with standards, buffers and detection reagents, the Protein A ELISA Kit streamlines assay set-up so you can focus on results. Choose the Protein A ELISA Kit from Assay Genie for quality you can trust.