[KO Validated] CD133 Rabbit Polyclonal Antibody (CAB12711)

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SKU:
CAB12711
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
RP41, AC133, CD133, MCDR2, STGD4, CORD12, PROML1, MSTP061, 33
Research Use:
Cell Biology
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Description

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[KO Validated] CD133 Antibody (CAB12711)

The [KO Validated] CD133 Antibody (CAB12711) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is optimized for Western blot applications.PROM1, also known as CD133, is a marker for cancer stem cells and is linked to tumor initiation and progression in various types of cancer. By targeting PROM1, researchers can investigate its role in cellular processes such as proliferation, differentiation, and self-renewal, offering insights into cancer biology and potential therapeutic strategies.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] CD133 Antibody
SKU:CAB12711
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ANHQ VRTR IKRS RKLA DSNF KDLR TLLN ETPE QIKY ILAQ YNTT KDKA FTDL NSIN SVLG GGIL DRLR PNII PVLD EIKS MATA IKET KEAL ENMN STLK SLHQ QSTQ LSSS LTSV KTSL RSSL NDPL CLVH PSSE TCNS IRLS LSQL NSNP ELRQ LPPV DAEL DNVN NVLR TDLD GLVQ QGYQ SLND IPDR VQRQ TTTV VAGI KRVL NSIG SDID NVTQ RLPI QDIL SAFS VYVN NTES YIHR NLPT LEEY DSY
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RP41, AC133, CD133, MCDR2, STGD4, CORD12, PROML1, MSTP061, 33
Positive Sample:HCT116
Cellular Localization:Apical Cell Membrane, Cell Projection, Endoplasmic Reticulum, Endoplasmic Reticulum-Golgi Intermediate Compartment, Multi-Pass Membrane Protein, Cilium, Microvillus Membrane, Photoreceptor Outer Segment.
Calculated MW:97kDa
Observed MW:120kDa

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID8842
RRIDAB_2759552
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HCT116 cells, using [KO Validated] CD133 Rabbit pAb (CAB12711) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of lysates from wild type (WT) and CD133 knockout (KO) HCT116 cells, using [KO Validated] CD133 Rabbit pAb (CAB12711) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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