Poly (ADP-Ribose) ELISA Kit (AEFI01026)

Poly (ADP-Ribose) ELISA Kit

The Poly-ADP-Ribose ELISA Kit is specifically designed for the precise measurement of Poly-ADP-ribose (PAR) levels in a variety of samples including cell lysates, tissue homogenates, and biological fluids. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for research applications. Poly-ADP-ribose is a critical molecule involved in DNA repair, genomic stability, and various cellular processes. Dysregulation of PAR levels has been implicated in diseases such as cancer, inflammatory disorders, and neurodegenerative conditions.

Therefore, monitoring PAR levels can provide valuable insights into disease mechanisms and potential therapeutic strategies. With the Poly-ADP-Ribose ELISA Kit, researchers can investigate the role of PAR in various physiological and pathological processes, ultimately advancing our understanding of disease mechanisms and fostering the development of targeted therapies. Trust in this kit to deliver reliable and actionable data for your research needs.

Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2	Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3	Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4	Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5	Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6	Reading: Measure absorbance at 450 nm using a microplate reader.