The POLD1 Monoclonal Antibody (CAB4218) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications.POLD1 plays a crucial role in maintaining genomic stability and preventing the accumulation of mutations that can lead to cancer development. Dysregulation of POLD1 has been implicated in various cancer types, making it a potential target for cancer therapy research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
POLD1 Monoclonal Antibody
SKU:
CAB4218
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0930
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC2, MDPL, POLD, CRCS10, POLD1
Positive Sample:
HeLa, A-549, Jurkat, Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
124kDa
Observed MW:
124kDa
This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.
Purification Method
Affinity purification
Gene ID
5424
RRID
AB_2863211
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POLD1 Rabbit mAb (CAB4218) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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