The POFUT2 Antibody (CAB12223) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the POFUT2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for research in the fields of glycobiology and protein modification.POFUT2 is essential for the glycosylation of certain proteins, playing a crucial role in various cellular processes including cell signaling and adhesion.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
POFUT2 Antibody
SKU:
CAB12223
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FUT13, C21orf80, POFUT2
Positive Sample:
U-87MG, HepG2, Mouse brain, Mouse heart, Rat brain, Rat heart
Cellular Localization:
Endoplasmic Reticulum, Golgi Apparatus.
Calculated MW:
50kDa
Observed MW:
50kDa
Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).
Purification Method
Affinity purification
Gene ID
23275
RRID
AB_2759102
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POFUT2 Rabbit pAb (CAB12223) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of H9C2 cells using POFUT2 Rabbit pAb (CAB12223) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
