The Phospho-STK3/MST2-T384 Antibody (CABP0935) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications.STK3, also known as MST2, is a serine/threonine kinase involved in various cellular processes, including cell proliferation, apoptosis, and cell migration. Phosphorylation of STK3 at threonine 384 has been linked to its activation and regulation of downstream signaling cascades.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-STK3/MST2-T384 Antibody
SKU:
CABP0935
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
NATS P
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KRS1, MST2, Phospho-STK3/MST2-T384
Positive Sample:
HeLa treated with Calyculin A, NIH/3T3 treated with Calyculin A
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
56kDa
Observed MW:
54kDa
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6788
RRID
AB_2863847
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-STK3/MST2-T384 Rabbit pAb (CABP0935) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
